Instrumental Methods of Analysis - for B. PHARMACY PCI SYLLABUS - 17 ( SEM VII - BP701T) - TEXTBOOK

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UNIT - I UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component analysis (Chapter - 1) Fluorimetry Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications (Chapter - 2) UNIT - II IR spectroscopy Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications (Chapter - 3) Flame photometry - Principle, interferences, instrumentation and applications (Chapter - 4) Atomic absorption spectroscopy - Principle, interferences, instrumentation and applications (Chapter - 5) Nepheloturbidometry - Principle, instrumentation and applications (Chapter - 6) UNIT - III Introduction to chromatography Adsorption and partition column chromatography - Methodology, advantages, disadvantages and applications. (Chapter - 7) Thin layer chromatography - Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications. (Chapter - 8) Paper chromatography - Introduction, methodology, development techniques, advantages, disadvantages and applications (Chapter - 9) Electrophoresis - Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications (Chapter - 10) UNIT - IV Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications

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Author: [Dr. Sandeep S. Sonawane, Parag A. Pathade, Dr. Sanjay J. Kshirsagar.] Pages: 244 Edition: 2022 Vendors: Technical Publications