Advanced Instrumentation Techniques - for B. PHARMACY PCI SYLLABUS - 17 ( SEM VIII - BP811ET)

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SYLLABUS Advanced Instrumentation Techniques (BP 811 ET) UNIT - I Nuclear Magnetic Resonance spectroscopy Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications. (Chapter - 1) Mass Spectrometry- Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications. (Chapter - 2) UNIT - II Thermal Methods of Analysis : Principles, instrumentation and applications of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC). (Chapter - 3) X-Ray Diffraction Methods : Origin of X-rays, basic aspects of crystals, Xray Crystallography, rotating crystal technique, single crystal diffraction, powder diffraction, structural elucidation and applications. (Chapter - 4) UNIT - III Calibration and validation - As per ICH and USFDA guidelines (Chapter - 5) Calibration of following Instruments Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC. (Chapter - 6) UNIT - IV Radio immune assay : Importance, various components, Principle, different methods, Limitation and Applications of Radio immuno assay (Chapter - 7) Extraction techniques : General principle and procedure involved in the solid phase extraction and liquid-liquid extraction (Chapter - 8) UNIT - V Hyphenated techniques - LC-MS/MS, GC-MS/MS, HPTLC-MS. (Chapter - 9)

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Pages: 282 Edition: 2023 Vendors: Technical Publications