Pharmaceutical Microbiology for B. PHARMACY PCI SYLLABUS - 17 ( SEM III - BP303T) - TEXTBOOK

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Unit I Introduction, history of microbiology, its branches, scope and its importance. Introduction to Prokaryotes and Eukaryotes Study of ultra-structure and morphological classification of bacteria, nutritional requirements, raw materials used for culture media and physical parameters for growth, growth curve, isolation and preservation methods for pure cultures, cultivation of anaerobes, quantitative measurement of bacterial growth (total and viable count). Study of different types of phase constrast microscopy, dark field microscopy and electron microscopy. (Chapters - 1, 2, 3, 4) Unit II Identification of bacteria using staining techniques (simple, Gram’s and Acid fast staining) and biochemical tests (IMViC). Study of principle, procedure, merits, demerits and applications of physical, chemical gaseous,radiation and mechanical method of sterilization. Evaluation of the efficiency of sterilization methods. Equipments employed in large scale sterilization. Sterility indicators. (Chapters - 5, 6, 7) Unit III Study of morphology, classification, reproduction/replication and cultivation of Fungi and Viruses. Classification and mode of action of disinfectants Factors influencing disinfection, antiseptics and their evaluation. For bacteriostatic and bactericidal actions Evaluation of bactericidal and Bacteriostatic. Sterility testing of products (solids, liquids, ophthalmic and other sterile products) according to IP, BP and USP. (Chapters - 8, 9, 10) Unit IV Designing of aseptic area, laminar flow equipments; study of different sources of contamination in an aseptic area and methods of prevention, clean area classification. Principles and methods of different microbiological assay. Methods for standardization of antibiotics, vitamins and amino acids. Assessment of a new antibiotic. (Chapters - 11, 12, 13) Unit V Types of spoilage, factors affecting the microbial spoilage of pharmaceutical products, sources and types of microbial contaminants, assessment of microbia

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Author: [Dr. Vimal Kumar, Sunil Kumar Ojha, Dr. Jaswandi S. Mehetre] Pages: 248 Edition: 2022 Vendors: Technical Publications